FastQCFastQC Report
Sun 7 Aug 2016
GBM129.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM129.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35106781
Sequences flagged as poor quality0
Sequence length83
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCGAT56350.010.0467621
CCCGTAT36750.09.1172671
CCCGGAT102550.08.6376371
TCGCATT55950.08.2558382
CGTATCG28700.07.91303973
CTCGCAT70050.07.75199941
CTCCGGT70450.07.65331841
CTCGGAT77900.07.56409071
CTCGTAT31700.07.410941
CTCCCGT107200.07.40074061
TCCGATT46100.07.34787562
CTCGTTT62650.07.3152411
CGGGGTT100800.07.25931841
GTATCGA29350.07.21318634
CCCGGTT58800.06.9427461
GTTCGAT53350.06.9301011
CTCGAAT58750.06.88310151
CCCCGGT96850.06.7203181
CCGATTA32700.06.70965673
TCGATCG24450.06.614358
Produced by FastQC (version 0.11.2)