FastQCFastQC Report
Sun 7 Aug 2016
GBM134.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM134.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43274219
Sequences flagged as poor quality0
Sequence length83
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT56900.013.021761
CGTATCG50400.012.7597143
GTATCGA44850.011.5054054
CCGTATC73650.09.8817312
CTACCGT45500.08.8865868
ATCGAAG113450.08.5879226
TACCGTA46150.08.4273239
CGCGTAA12101.8189894E-128.292181
TATCGAA76300.07.72202975
CGCGTTT19600.07.6787281
TCGAAGG132800.07.39448077
CCGGTAT76350.07.37945941
CGCCAAT55400.07.1747731
GTCCGTT36050.07.0651181
ACTACCG58550.07.03756577
CCGGCAT86250.07.02459531
TCGCATT62050.06.95056722
GCCGTAT48000.06.67294651
CGATTAG48600.06.65583564
CCGGGAT124150.06.558681
Produced by FastQC (version 0.11.2)