FastQCFastQC Report
Sun 7 Aug 2016
GBM137.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM137.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48283579
Sequences flagged as poor quality0
Sequence length83
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT64500.012.58970451
CGTATCG58600.011.5559643
GTATCGA53500.011.1472394
TACCGTA56150.010.0729149
CTACCGT60200.010.0343848
CGACTAA65550.09.74646428-29
CCGTATC79600.09.5229762
CGACCGT35450.09.4448661
ATCGAAG132100.08.1262276
ACTACCG71500.07.4260977
CGGGGTT138300.07.2629181
CGGGTGT121750.07.2070691
CCCGGAT136800.07.20189331
CCCGGTT66300.07.13977241
GTTCGAT77200.07.12872031
CAATCGG64350.07.11518149
CGCGTAA12007.365088E-97.0556051
GTCCGAT30250.06.99729441
TCAATCG66600.06.9325758
CTCCGAT100750.06.91395141
Produced by FastQC (version 0.11.2)