FastQCFastQC Report
Sun 7 Aug 2016
GBM137.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM137.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48283579
Sequences flagged as poor quality0
Sequence length83
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGA53550.013.15426354
CCCGTAT69200.012.9674781
CGTATCG60500.012.2794053
CCGTATC88600.09.7319342
CGACTAA67250.09.30094328-29
ATCGAAG130350.08.7442136
CGCGAAT13950.08.2823341
CTCCGAT102000.07.7025711
TATCGAA94800.07.5929955
CGACCGT35050.07.4718231
TCCGATT79400.07.417472
CGACGTT48950.07.39180563
CCCGGAT144800.07.28765341
TCGAAGG154550.07.1756947
CGGGGAT141100.06.98744871
CTACCGT56750.06.85324058
CGGGTGT122250.06.8362291
CTCGGTT67100.06.77275231
CGGGGTT143800.06.74912361
GTCCGAT29800.06.7203641
Produced by FastQC (version 0.11.2)