FastQCFastQC Report
Sun 7 Aug 2016
GBM14.R1.fastq.83bp.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM14.R1.fastq.83bp.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33448665
Sequences flagged as poor quality0
Sequence length83
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG29750.011.9017053
CCCGTAT37800.010.8956271
CGCGTAA11150.010.3563491
CTCGGTT48000.010.2642921
CTCCGAT51900.09.4929871
TTCGCGC24600.09.2299757
GTATCGA32100.08.9918614
CTCGAAT65700.08.9050941
CTCGGAT77900.08.8939641
CTCGCAT79600.08.8007281
ATCGAAG96850.08.7421536
CCGTATC44750.08.514562
CTGGAAT322150.08.5071121
CTCAGAT204250.08.404911
CTCAAAT214900.08.2212261
TGCGCGA27650.08.2118539
CCGTACG35600.07.99972634
TCGCATT62900.07.9544972
TCGATCG39350.07.92181258
CTTGGAT204800.07.7809151
Produced by FastQC (version 0.11.2)