FastQCFastQC Report
Sun 7 Aug 2016
GBM148.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM148.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70581861
Sequences flagged as poor quality0
Sequence length83
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT85200.013.510271
CGTATCG72500.013.3280973
GTATCGA75700.011.59501654
CCGTATC113400.09.6752962
CCCGGAT219150.08.3266311
CTCGTAT69200.07.95540671
CTCGGAT167850.07.82105731
ATCGAAG191250.07.6895156
CTCCGAT135200.07.68810841
CCGGGAT207700.07.45112751
CGGGGTT213750.07.4383461
CGCGAAT21300.07.41031551
GTCCGAT62250.07.2975171
CCGGTAT106050.07.2602531
CGACTAA85700.07.232310328-29
TCGCATT99200.07.21828032
CCGGAAT128400.07.1957931
CCCGGTT120300.07.13627671
CGGGGAT220150.07.0996971
TATCGAA125450.07.0888165
Produced by FastQC (version 0.11.2)