FastQCFastQC Report
Sun 7 Aug 2016
GBM15.R2.fastq.83bp.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM15.R2.fastq.83bp.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53242722
Sequences flagged as poor quality0
Sequence length83
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG33700.010.9607683
CTCGGAT120300.010.427941
CCCGTAT58800.010.07836151
GACGCTA42400.09.437734
CTCCGAT94100.09.3646691
CTTGGAT337950.08.8360021
CTCAGAT348600.08.8309851
CTGGAAT486500.08.7561131
CTCGTAT49950.08.5513371
GTATCGA52100.08.4191294
CTCGAAT105200.08.3399931
CTCAAAT367950.08.2097021
CTCGGTT81450.08.1733771
TACCGTA38600.08.0772929
CTCGGGT116000.08.061111
CAATCGG55400.07.9176294
CTCCGGT95200.07.88214161
CTCGCAT89950.07.8716031
AATCGGG66100.07.85809475
CGCGAAT16250.07.81460671
Produced by FastQC (version 0.11.2)