FastQCFastQC Report
Sun 7 Aug 2016
GBM155.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM155.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35009954
Sequences flagged as poor quality0
Sequence length83
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT39050.010.6511011
CGTATCG30550.09.9538523
GTATCGA30950.08.332774
CGGGGTT97700.08.0413221
CCCGGAT98350.07.83154541
CTCGCAT75250.07.6255571
CCGGTAT64700.07.6189921
CCGTATC52700.07.4503072
CTCCGGT62500.07.3326121
CGCCAAT46600.07.1072951
TTCGCGC26250.07.0411097
TCCGATT50300.06.9639882
CTCCGAT62250.06.86713271
CGTCGAT25250.06.86345671
ATCGAAG80250.06.8615176
CGCCTAT26200.06.76158241
CCCGGGT138450.06.75935271
CGCGAAT9151.2064513E-56.73427251
CCCGAAT77050.06.6976731
CCGGGAT103650.06.6508251
Produced by FastQC (version 0.11.2)