FastQCFastQC Report
Sun 7 Aug 2016
GBM156.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM156.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43436769
Sequences flagged as poor quality0
Sequence length83
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG39750.09.2922013
CCCGTAT42000.09.071341
TTACGAC32400.07.7187485
GTTACGA32750.07.6363184
CTCGCGT29900.07.59392451
GTATCGA36800.07.42322354
TTATCCG55650.07.3285698
GCCGTAT43750.07.30105351
TACGCAC46300.07.234558650-51
CCGGTAT88400.07.22672131
CGCGTAT10301.8867286E-77.09907631
CGCAGTT93150.07.0234671
CTCCGAT68600.07.0124761
CCGTATC59150.06.9605092
ATCGAAG112300.06.9207016
CCGGCAT89750.06.903621
CGGGGTT119250.06.7771391
CCGGGAT131300.06.7120661
TGTTACG35600.06.70079233
CCCGGTT66650.06.6979721
Produced by FastQC (version 0.11.2)