FastQCFastQC Report
Sun 7 Aug 2016
GBM156.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM156.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43436769
Sequences flagged as poor quality0
Sequence length83
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT47350.010.7358231
GTCCGAT32850.08.5579331
CCCGGAT143000.08.2676821
CGTATCG43650.08.2011463
GTTACGA32900.07.95589164
AGTACGC47850.07.602601548-49
CGCCAAT56100.07.34517771
TTACGAC34800.07.3004075
TACGCAC47700.07.26334250-51
CCGTATC70000.07.25875662
CTCGCGT32700.07.0661831
CTCGGTT63950.07.0457411
TTATCCG58150.07.0191828
CTCCGAT69750.06.9567711
CTCGGAT100950.06.904841
CGATTAG48600.06.8114264
CTCCGGT92350.06.79723171
ATCGAAG113000.06.7811756
TTCGCGC38100.06.7714277
CGCAGTT95200.06.7151011
Produced by FastQC (version 0.11.2)