FastQCFastQC Report
Sun 7 Aug 2016
GBM16.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM16.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20091558
Sequences flagged as poor quality0
Sequence length83
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCTA10200.011.6922734
TCGTATG19350.010.86004742-43
CGTATCG23900.010.3023883
CCCGTAT23950.010.2828761
GTATCGA18950.09.7447224
CGTATGC22550.09.5973244-45
GATCGGA45450.09.0592041
TATGCCG23550.08.94397146-47
TTCGCGC17100.08.7738847
TCGCGCG7506.712435E-88.7198918
ATCGGAA51700.08.4091972
GTTACGG35950.08.3467837
GCGAACG9051.9394065E-88.0772013
GTCTAAC29550.07.94163274
TACGGAT44100.07.93827639
CTCGGTA25350.07.89342551
CCGTATC34300.07.85182432
CGCTAAT7901.3628178E-67.79350951
TTACGAC12352.7284841E-117.78747135
CTCGTAT26050.07.77083642-43
Produced by FastQC (version 0.11.2)