FastQCFastQC Report
Sun 7 Aug 2016
GBM28.R1.fastq.83bp.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM28.R1.fastq.83bp.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84873305
Sequences flagged as poor quality0
Sequence length83
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG56650.010.3262993
CCCGTAT93800.09.643591
CTCGGAT198350.09.353811
TACCGTA76350.09.173599
GTATCGA79300.09.075454
CTGGAAT794150.09.0202281
CTTGGAT547350.08.8398291
CTCAGAT541700.08.7543841
CTCCGAT135200.08.6265991
CTCAAAT570550.08.4938731
CTCGAAT163450.08.171811
CGACCTA46100.08.0978654
CTCGGTT133250.07.97288661
CTCGCAT162100.07.9074221
CCGGGAT188100.07.87853861
CTCGTTT158550.07.8416961
CTGAGAT691550.07.76469331
CGGGGAT187700.07.75177671
CTTGAAT522000.07.7279531
CTCGGGT177100.07.6723751
Produced by FastQC (version 0.11.2)