FastQCFastQC Report
Sun 7 Aug 2016
GBM28.R2.fastq.83bp.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM28.R2.fastq.83bp.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84873305
Sequences flagged as poor quality0
Sequence length83
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG56750.010.7804983
CCCGTAT95700.010.25377751
CTCGGAT199550.010.1049751
CTCCGAT141100.09.9000021
CTCGGTT131000.09.6938941
CGCGAAT27400.09.409791
CTCAGAT552150.09.143921
CTCGAAT158950.09.1271771
CTGGAAT790000.08.9872111
CTCGTAT82100.08.9056611
CTTGGAT545600.08.8657721
CTCGCAT164400.08.8480111
GTATCGA76700.08.7289394
CTCAAAT579600.08.4851231
CTCCGTT144050.08.3348351
CTCGTTT167050.08.177821
TACCGTA73150.08.1557459
CTCGAGT155700.08.0077781
CTGAGAT691000.07.9581091
CTCGGGT178150.07.81948951
Produced by FastQC (version 0.11.2)