FastQCFastQC Report
Sun 7 Aug 2016
GBM3.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM3.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55840381
Sequences flagged as poor quality0
Sequence length83
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG50850.014.8304173
CCCGTAT65550.013.9146411
GTATCGA51300.013.2752344
CCGTATC78850.010.8334392
ATCGAAG120100.08.8099786
CTACCGT61650.08.7373578
GTCCGAT36300.08.5876591
GTCTAAC83900.08.0711794
TATCGAA85550.08.0504125
TACCGTA63500.07.8768899
CTCCGAT100700.07.8728881
CGATTAG60350.07.4592454
TCCGATT88400.07.31260442
GTTCGAT86750.07.23126841
ACTACCG75450.07.1902677
CGGGGTT150450.07.188041
GTCGGTT87950.07.1763621
CCCGGAT141150.07.170861
GTCCGTT44650.07.06787351
CGACCGT36400.06.8723991
Produced by FastQC (version 0.11.2)