FastQCFastQC Report
Sun 7 Aug 2016
GBM3.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM3.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55840381
Sequences flagged as poor quality0
Sequence length83
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT70550.013.756611
CGTATCG51750.013.0165633
GTATCGA52150.012.4738544
CCGTATC86750.09.1407442
CTCCGAT101700.08.9750171
TATCGAA86450.08.4153335
ATCGAAG122000.08.3640186
CGACCGT34350.07.96048451
CTCGGAT119600.07.72837071
GTTCGAT89550.07.5692871
CCCGGAT145100.07.4584191
CGATTAG60550.07.37415274
TCCGATT83200.07.356272
CTCCGGT116850.07.18514731
CCCCCGT129300.07.17837951
CCGGTAT97150.07.09607361
CCCGGTT92650.06.98347761
CCGATTA69050.06.968113
CCCCGGT152550.06.84171441
CTACCGT58900.06.73365458
Produced by FastQC (version 0.11.2)