FastQCFastQC Report
Sun 7 Aug 2016
GBM38.R2.fastq.83bp.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM38.R2.fastq.83bp.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31631370
Sequences flagged as poor quality0
Sequence length83
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG24950.015.5774253
CCCGTAT40350.014.0208681
GTATCGA33850.012.0501384
CCGTATC42700.010.8142952
CTCGGAT65450.010.1727311
CAATCGG35000.09.6751894
CTTGGAT196000.09.1305721
CTCCGAT52900.09.0212591
TCAATCG38750.08.8381863
CTGGAAT292500.08.6971451
AATCGGG42100.08.6831115
CCCGTCT113200.08.6695171
CTCAGAT203900.08.6635641
GACGCTA26400.08.599894
ATCGAAG68000.08.5472586
CTCGTCT93200.08.4239431
CTCGACT37750.08.3598411
CTCGGTT47250.08.3080571
TATCGAA51050.08.2914555
ATTCGCG7102.4921083E-68.1318946
Produced by FastQC (version 0.11.2)