FastQCFastQC Report
Sun 7 Aug 2016
GBM40.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM40.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35270278
Sequences flagged as poor quality0
Sequence length83
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT37650.013.3948481
CGTATCG31350.011.2974843
GTATCGA30600.010.8195484
CTCCGGT69100.08.6911661
CCGTATC50000.08.469432
CCCGGAT94300.08.2057041
GTCCGAT23750.08.1047161
CTCGTAT31800.07.86896661
CGGGGTT100400.07.8221821
CTCGGAT74350.07.81857161
CGCGTAA9001.4961734E-77.69948151
CCCCGGT94200.07.5605311
CTCCGAT73600.07.5321011
CGACTAA40750.07.463284528-29
CGACCGT24150.07.332841
CTCGGTT51050.07.31488371
GTCCGTT27900.07.31312751
CCCCCGT84550.07.2851391
CTCGCGA15950.07.2408911
TACCGTA34700.07.2114019
Produced by FastQC (version 0.11.2)