FastQCFastQC Report
Sun 7 Aug 2016
GBM43.R1.fastq.83bp.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM43.R1.fastq.83bp.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53527052
Sequences flagged as poor quality0
Sequence length83
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG48150.014.863113
CCCGTAT72500.012.3679621
GTATCGA59250.012.3383374
CCGTATC75550.010.8477122
TACCGTA48000.010.5007689
TATCGAA83400.09.5959655
CTCGGAT124000.09.1554691
ATCGAAG121900.08.6800296
CTACCGT61650.08.4254238
CTCAGAT328650.07.89235351
CTTGGAT326600.07.8594091
GACGCTA48700.07.742634
CTGGAAT485950.07.67383431
CGCGTAA11151.6789272E-97.59325651
ACTACCG79050.07.5443327
CCCGTCT184050.07.4437961
CGGGGTT144450.07.43304731
CTCGTTT93900.07.41810941
AATCGGG67050.07.4025675
CTCCGAT90050.07.39337351
Produced by FastQC (version 0.11.2)