FastQCFastQC Report
Sun 7 Aug 2016
GBM43.R2.fastq.83bp.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM43.R2.fastq.83bp.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53527052
Sequences flagged as poor quality0
Sequence length83
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG49250.014.5310213
CCCGTAT73350.013.7969541
GTATCGA62350.012.8353774
CCGTATC82350.010.7928812
CTCGGAT119950.09.9767181
CTCCGAT95250.09.37239551
CGCGTAT11000.09.0951171
TATCGAA84500.08.9235265
CTTGGAT328800.08.8708451
ATCGAAG121150.08.8052826
CTCGGTT81750.08.5195851
CTCGAAT105050.08.49805451
CTCAGAT323600.08.4069511
CTCGCAT100350.08.3208931
CTGGAAT475600.08.2929611
TCGGATT71650.08.2160732
CTCGTAT46350.08.1358691
CTCGTTT92250.08.050421
TACCGTA40900.07.81517749
TAAGGCG77400.07.7121968
Produced by FastQC (version 0.11.2)