FastQCFastQC Report
Sun 7 Aug 2016
GBM44.R1.fastq.83bp.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM44.R1.fastq.83bp.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences699621
Sequences flagged as poor quality0
Sequence length83
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTACG1650.032.6011963
GTTACGA1800.032.019034
TGCCGTA1101.2836608E-831.4368692
TTACGAC1850.031.1536525
CCGAAAG1001.944245E-730.7538381
GCCGTAT1251.3114914E-930.7382683
CCACGAG1052.981178E-729.2893711
TCCGCTT804.5905792E-528.8171272
ACGAGCG1104.498561E-727.9438843
CGGAAAC1456.364644E-926.511931
CGCCAGA1059.859148E-625.62821
CGTATCG1509.1822585E-925.6152275
ATCCGGT2100.025.6152276
TCCCGTC1059.8979635E-625.6152272
ACTATGC2400.025.6152255
CACGAGC1209.616851E-725.6152252
TTATCCG2300.025.0583744
CGGTAAA2151.8189894E-1225.0213329
AGGCGTA1551.3005774E-824.790728
TACGACT2350.024.5252156
Produced by FastQC (version 0.11.2)