FastQCFastQC Report
Sun 7 Aug 2016
GBM6.R1.fastq.83bp.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM6.R1.fastq.83bp.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83439125
Sequences flagged as poor quality0
Sequence length83
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG66150.013.7874833
CCCGTAT98850.011.9942481
TGCGCGA73750.011.1661299
GTATCGA80850.010.0428864
CTCGGAT186550.09.7809731
CTCGCAT180800.09.7726711
CCGTATC111550.09.7636112
CTCCGAT152450.09.6204761
CTCGGTT119550.09.3378611
CCGGTAT122050.09.178131
CAATCGG90200.09.1298444
CTCAGAT525800.09.1148131
CTTGGAT536400.08.9705741
TCGCATT127850.08.9101412
CTGGAAT752900.08.8707671
ACGTGCG116000.08.7579736
CTCGAAT159050.08.73720551
CTCGTTT156250.08.6720481
TCGATCG58750.08.6460348
ATCGAAG211100.08.3307796
Produced by FastQC (version 0.11.2)