FastQCFastQC Report
Sun 7 Aug 2016
GBM6.R2.fastq.83bp.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM6.R2.fastq.83bp.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83439125
Sequences flagged as poor quality0
Sequence length83
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT94650.012.3971531
CGTATCG77950.011.39891053
TGCGCGA69100.011.091359
CTCCGAT152650.011.0135521
CTCGGAT188700.010.9074851
CTCGTAT80500.010.8963671
CTCGGTT127200.010.1018781
GTATCGA83400.09.8228864
CTCAGAT522250.09.8048821
CTCGCAT182750.09.7679481
CTCGAAT166500.09.4966481
TCGATCG64200.09.418368
CTTGGAT540050.09.3891051
CAATCGG95100.09.2614834
ACGTGCG110500.09.1972986
ATCGAAG211500.09.1553766
CTGGAAT765450.09.0971321
CTCGTTT159200.08.8929831
CTCCGTT170000.08.8258891
CGATCGG68900.08.7758629
Produced by FastQC (version 0.11.2)