FastQCFastQC Report
Sun 7 Aug 2016
GBM61.R1.fastq.83bp.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM61.R1.fastq.83bp.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1326109
Sequences flagged as poor quality0
Sequence length83
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCCG150.001796320373.8802848-49
TGCCGTA2050.039.2726362
TCAGACG2400.036.594962
CCGAAAG2250.035.9107971
TTATCCG3350.034.9563754
ATCCGGT3250.034.905966
AGGCGTA2950.033.493698
CCGGTAA3550.032.9870038
CGTCAGT450.003403625732.646181
ACTCGTA450.00346418632.528858
CCTCACC12950.031.7638511
CGGTAAA3600.031.5135159
TATCCGG3600.031.5123235
CGTAGAC702.5125979E-531.4802461
TCCGGTA3750.031.2276957
CAGGCGT3050.031.1957047
GCCGTAT2750.030.6066913
TGTTACG3600.030.4957963
CTGCCGT2900.030.394721
CGCCAGA852.720877E-630.2457261
Produced by FastQC (version 0.11.2)