FastQCFastQC Report
Sun 7 Aug 2016
GBM61.R2.fastq.83bp.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM61.R2.fastq.83bp.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1326109
Sequences flagged as poor quality0
Sequence length83
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGCG2150.039.2202077
CCTGCCA3250.037.2377171
TGCCGTA2200.036.6554152
TCCCGTC1850.035.6647262
GGCTCGT2450.034.4164284
CTGCCGT2500.033.7396281
CTCGAAC552.2759449E-433.3395541
TGTTACG3350.032.8319743
ATCGGGA901.1726661E-732.582592
CCGGCAT852.7519345E-630.2017141
GTCCCGT2450.029.927336
GTGCGTC2700.029.8798489
CTCGCCA754.0646402E-529.3388081
ACCCGAA1002.9487455E-729.3298977
CCTCACC13050.028.9453741
CTCACTA5200.028.196472
GTTACGA3950.027.8437844
GTTAGGA1204.712274E-827.505131
CACTAAA5550.027.0828864
GCTCGTG3000.026.8847185
Produced by FastQC (version 0.11.2)