FastQCFastQC Report
Sun 7 Aug 2016
GBM63.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM63.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28109839
Sequences flagged as poor quality0
Sequence length83
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA78200.014.3697241
TCGTATG42300.013.56498244-45
CGTATGC44000.012.77789846-47
TATGCCG44450.012.60365348-49
ATCGGAA93350.012.1172952
CTCGTAT49350.011.23564244-45
CGTATCG23500.010.9689073
TCGGAAG119600.09.939943
CCCGTAT28650.09.9398411
GTATCGA24000.08.8162364
CTCGGAT59100.08.6603711
TCTCGTA70300.08.44617342-43
ATGCCGT74300.08.24215248-49
CGGAAGA153350.08.2034354
CCGTATC34550.07.9064762
TCGGATT39450.07.8997022
GTCGGAT27300.07.8940111
CGCGTAT4900.00183093797.85373541
CTCCGAT40450.07.80131241
CCGGTAT48450.07.7840331
Produced by FastQC (version 0.11.2)