FastQCFastQC Report
Sun 7 Aug 2016
GBM63.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM63.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28109839
Sequences flagged as poor quality0
Sequence length83
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG23600.011.0924743
CCCGTAT29900.010.944081
CGCGTAT5801.4327688E-69.2924791
GTATCGA23450.08.8650764
CCCGGTT46000.08.7874531
CCCGGAT78300.07.9649821
CTCGGTT40450.07.5186541
CGACCGT20150.07.2600581
CTCGGAT58150.07.21619561
CTCGCAT53350.07.2160091
CCCGGGT103850.07.154551
CCGTATC44950.07.10853152
ATCGAAG65000.06.9888356
CGGGGTT82250.06.9271931
CGCGTAA9553.1133077E-66.85294251
CCCGCAT56800.06.84549051
CTCCGAT42750.06.75393151
CGCCAAT31500.06.72177031
CGCGTTA26000.06.664886534-35
CCGGAAT43350.06.66045141
Produced by FastQC (version 0.11.2)