FastQCFastQC Report
Sun 7 Aug 2016
GBM64.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM64.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82564754
Sequences flagged as poor quality0
Sequence length83
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT107000.013.23881151
CGTATCG83750.012.9435953
GTATCGA91250.011.7106244
CCGTATC130950.09.2207992
CGCGTAT18850.09.0096531
CGACCTA44200.08.46608454
ATCGAAG207650.08.360166
CTCGGTT121300.07.60508061
TATCGAA160550.07.6001535
CCCGGTT123850.07.5419931
GTCCGAT54200.07.4062911
CGCGAAT21900.07.4023891
CGACTAA100700.07.396252628-29
CCCGGAT228450.07.36651471
CTCGGAT181450.07.14741561
CGGGGTT220350.07.0592531
CCGTAAT76050.07.0547611
CCGGGAT218900.06.98258351
CGACCGT59750.06.97674041
CCCGGGT311250.06.90736771
Produced by FastQC (version 0.11.2)