FastQCFastQC Report
Sun 7 Aug 2016
GBM64.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM64.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82564754
Sequences flagged as poor quality0
Sequence length83
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG88000.012.4278133
CCCGTAT113350.011.5417611
GTATCGA92900.010.6947444
CGACCTA44500.09.519174
CCGTATC138650.09.1651922
CCCGGAT238750.08.518461
CGACTAA108000.08.39513228-29
CCCGGTT135000.08.3472411
CTCCGAT168100.08.0581111
CTCGGTT123300.07.699561
CTCGGAT188850.07.6222951
CGACCGT56950.07.5895871
ATCGAAG214900.07.47276456
CCGGTAT126200.07.33915041
TCCGATT127550.07.3362352
CGCGAAT21700.07.2915191
CCCGAAT196950.07.2892161
CCGGGAT233250.07.2137111
CGCGATT22350.07.0794611
GTCCGAT53550.06.9904661
Produced by FastQC (version 0.11.2)