FastQCFastQC Report
Sun 7 Aug 2016
GBM76.R1.fastq.83bp.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM76.R1.fastq.83bp.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58615805
Sequences flagged as poor quality0
Sequence length83
%GC49

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT57150.011.1456841
ATCGAAG143500.09.616746
CGCGTAA28200.09.1395711
CGTATCG42300.08.936973
CCGACAT130650.08.9054231
TCGATCG53200.08.623598
TCGCATT94100.08.2687612
CTCGCAT124000.08.1359021
CCGGTAT89900.08.1092821
TCGAAGG173600.08.0761327
CCGTATC70550.07.9075282
CTCAGAT346300.07.84658531
CTCGTAT57500.07.74808551
CTCGGTT88550.07.6507871
CTGGAAT505150.07.6022271
CTCCGAT87900.07.58169941
CCGCAAT52300.07.53284361
CTCGGAT126700.07.52662041
GTATCGA47950.07.50117734
CTCGAAT107100.07.49452071
Produced by FastQC (version 0.11.2)