FastQCFastQC Report
Sun 7 Aug 2016
GBM80.R1.fastq.83bp.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM80.R1.fastq.83bp.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences103535978
Sequences flagged as poor quality0
Sequence length83
%GC48

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT117100.012.5154241
GTATCGA96850.011.48726754
CGTATCG113950.011.4390093
ATCGAAG267750.011.2861116
CCGGTAT157700.011.184671
CGCGTAA62350.010.8667761
CCGACAT218900.09.6893831
TCGAAGG302200.09.6593237
TGCGCGA103300.09.5614989
CCGTATC163600.09.4038372
CTCGGAT229450.09.2759661
CGGTATT181000.08.8446842
TATCGAA145700.08.3918695
CTCGGTT150650.08.2860961
ACGTGCG166500.08.11535556
CTCGCAT249050.08.0728241
GCGTAAC90500.07.99267242
CGCGTAT20000.07.9169491
ACATCGA274400.07.7609994
CGGGGTT235250.07.68548351
Produced by FastQC (version 0.11.2)