FastQCFastQC Report
Sun 7 Aug 2016
GBM80.R2.fastq.83bp.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM80.R2.fastq.83bp.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences103535978
Sequences flagged as poor quality0
Sequence length83
%GC48

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT116350.012.4683951
GTATCGA94900.011.7640864
CCGGTAT154150.011.2931391
CGTATCG124350.011.25208953
ATCGAAG261150.011.0959946
CGCGTAA60900.010.0108431
CCGACAT220150.09.5590731
TGCGCGA90550.09.4913169
CGGTATT175350.09.34069252
CCGTATC176550.09.2564042
TCGAAGG303550.09.2078427
CTCCGAT174750.09.1212091
CTCGCAT253950.09.0966671
CTCGGAT235100.09.0293211
CTCGGTT154500.08.4625531
TATCGAA147000.08.34445
CTCGTAT92000.08.2634691
TTCGCGC80500.08.2582147
CGCCAAT124800.08.2104991
TCGCATT180700.08.0479822
Produced by FastQC (version 0.11.2)