FastQCFastQC Report
Sun 7 Aug 2016
GBM81.R1.fastq.83bp.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM81.R1.fastq.83bp.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18552603
Sequences flagged as poor quality0
Sequence length83
%GC46

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTA19100.016.3344659
CGTATCG17150.016.265643
CCCGTAT29100.014.0397721
CCGTATC25250.013.3735942
GTATCGA22250.013.3620894
CTACCGT25200.013.2544058
CGCGTAT3505.6660247E-712.6195331
GCGAACG5852.4974725E-910.6663243
ACTACCG32200.010.2590237
ATCGAAG44250.09.78786856
CTCCGAT32600.09.7098151
CTCGTTT28950.09.5354841
CGCGTAA7156.3331754E-88.7513081
CCGCAAT17300.08.7230391
CCGGTAA12400.08.6080821
GACGCTA22750.08.3896024
TAAGGCG35550.08.3630518
CCCGGTT20550.08.239031
CGACCTA9004.6984496E-98.1565564
CCCGGAT35650.08.15638351
Produced by FastQC (version 0.11.2)