FastQCFastQC Report
Sun 7 Aug 2016
GBM84.R2.fastq.83bp.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM84.R2.fastq.83bp.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2254234
Sequences flagged as poor quality0
Sequence length83
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCA82270.364957675201421No Hit
CCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAA49680.22038528387026368No Hit
AGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCATAG42610.18902208022769595No Hit
CTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGC31890.14146712364377434No Hit
CTGGCTTGTGGCGGCCAAGCGTTCATAGCGACGTCGCTTTTTGATCCTTC31840.14124531880896127No Hit
GTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATCGAAGGATCAAAA26320.11675806504559864No Hit
GTGAAAGCGGGGCCTCACGATCCTTCTGACCTTTTGGGTTTTAAGCAGGA25720.11409640702784182No Hit
CAGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCATA24710.10961594936461785No Hit
CACGATCCTTCTGACCTTTTGGGTTTTAAGCAGGAGGTGTCAGAAAAGTT23560.10451443816391733No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACGGC2500.057.2178969
AGAGCGT2100.048.9041828
CAAGCAG3050.048.092811
TCGGAAG2900.046.78583
CAGAAGA4300.046.0526925
GATCGGA3300.045.5606541
CCTGCCA3500.042.9571881
CCGGCAT1150.041.453911
ATCGGGA1600.041.2536932
GACCGGA456.994074E-540.744392
GTTAGGA2100.040.163221
TTCGGTC653.381465E-739.490712
TCAGACG8350.039.0853352
TGCCGTA6400.037.8158842
CTGCCGT6750.037.4856761
CGGAAAG2450.037.419995
CAGTTTT9650.037.239953
ACGTATT200.001914945836.7105320-21
ATCATTA2150.036.38675752-53
TCTGGCG1152.3646862E-1135.081097
Produced by FastQC (version 0.11.2)