FastQCFastQC Report
Sun 7 Aug 2016
GBM85.R2.fastq.83bp.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM85.R2.fastq.83bp.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85402425
Sequences flagged as poor quality0
Sequence length83
%GC48

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACGGC283550.025.5777659
AAGACGG311350.023.3765358
CATACGA118650.023.37442414-15
GCATACG140250.022.62937514-15
CGGCATA149600.020.93212-13
ATACGAG133050.018.7471716-17
GAAGACG384650.018.6928677
CAAGCAG824900.013.3186951
ACGGCAT234700.013.26099910-11
GACGGCA281750.011.38540810-11
CCCGTAT95350.011.2836161
GGCATAC295950.011.033957512-13
TACGAGA183600.010.72439916-17
GATCGGA81750.010.2411441
CGCGTAA34800.010.1296251
AGAAGAC775350.010.0449746
GTATCGA73550.09.8846474
AAGCAGA1078050.09.7886762
CGTATCG80100.09.6723153
ATCGAAG192300.09.3757966
Produced by FastQC (version 0.11.2)