FastQCFastQC Report
Sun 7 Aug 2016
GBM10.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM10.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5458952
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGAC7450.025.1992035
GTTACGA7550.023.6217354
TGTTACG7850.023.3192123
TACGACT8850.022.2927346
TTATCCG8750.020.9467688
CGTATCG7450.018.900963
TTCGCGC9400.017.4984937
TATCCGG10450.017.0894379
TCACCCG31900.015.3024723
ACGACTT12400.015.1599867
CCGTATC10650.014.543982
GCCGTAT7750.013.9323581
AGGCGTA12000.013.315528
CCCGTAT6351.0913936E-1113.3075261
TCGTGCG8650.013.0393397
TATCGTT8350.012.9277955
CACCCGG37350.012.6912944
GATCGGA7501.8189894E-1212.5189311
GTATCGT9050.012.4462294
TAGCATA17600.012.2830349
Produced by FastQC (version 0.11.2)