FastQCFastQC Report
Sun 7 Aug 2016
GBM102.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM102.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences103973159
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG86300.013.5089383
CCCGTAT141250.011.7682051
GTATCGA103350.011.5806714
TACCGTA98100.010.1719759
CTCGGAT235350.010.0576021
CTCGGTT194600.09.5897361
CTCAGAT755950.09.3350661
CTCCGAT234950.09.2823311
CTTGGAT731400.09.2787111
CCGTATC162000.09.2484982
CTCGCAT227250.08.9726631
CTGGAAT1051850.08.689651
CTCGTAT117650.08.6280161
CTCGAAT229100.08.6099831
CTCGTTT215750.08.6085621
CTCAAAT773950.08.5509491
TAAGGCG177600.08.4030418
CCCGTCT440850.08.3153791
ATCGAAG247300.08.3000976
CTACCGT122600.08.2472238
Produced by FastQC (version 0.11.2)