FastQCFastQC Report
Sun 7 Aug 2016
GBM108.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM108.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60267933
Sequences flagged as poor quality0
Sequence length101
%GC48

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTGCGT38551.1277734E-1018.26172844-45
TCGCGAG22752.3867302E-517.68330646-47
ATCGCGA23953.710518E-516.79729346-47
CGCGATT15652.682047E-516.06840748-49
CGATTAC39154.9240043E-815.41581948-49
CTATCGA31052.0774092E-514.57535544-45
CGAATTA38957.797262E-714.20372948-49
CGTTAGG32453.1432097E-513.94906148-49
CGTTATA33304.008956E-513.59300348-49
TCGCGAT22400.00766881913.46970746-47
TTAGCGA45052.746001E-713.394443544-45
CGTATCG39100.013.1671093
TGCGTTA43602.7765363E-612.68706246-47
CGACTAT39701.4779158E-512.66854248-49
ATTTCGT84550.012.48946744-45
TATTCGT52501.2987948E-712.45151844-45
TTTCGCA64951.0550139E-912.38736944-45
TTAACGC32555.038468E-412.35882644-45
CTATCGT37501.2213891E-412.06839444-45
TTTCGTA87550.012.061501544-45
Produced by FastQC (version 0.11.2)