FastQCFastQC Report
Sun 7 Aug 2016
GBM108.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM108.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60267933
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG41450.014.5374013
GTATCGA58000.010.8476944
CCCGTAT81200.010.6400791
CTCGGAT138700.09.8068181
CTCCGAT115700.09.2671931
CTCGTAT65400.08.6038371
CTCAGAT429700.08.4550221
CCCGTCT235750.08.3256511
CTGGAAT604100.08.3170721
CTCGCAT112350.08.2815661
CTTGGAT401950.08.2010061
ATCGAAG134000.08.2009386
CCGTATC87000.08.0973782
CCCGGAT160350.07.98537061
CTCGTTT116350.07.95877271
GTCGAAT49100.07.9408571
CGGGAAT114100.07.68857341
CTCGAAT121500.07.65789181
CGCAGAT104700.07.61714171
TATCGAA90550.07.53545955
Produced by FastQC (version 0.11.2)