FastQCFastQC Report
Sun 7 Aug 2016
GBM116.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM116.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39608215
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCGCG13054.6875357E-934.8665544-45
CGCGATT12008.7055014E-833.70975548-49
CGATCGT10003.238651E-530.33389744-45
TTCGCGA19656.4411324E-620.58339946-47
TTGCGAC24951.10414476E-720.26312444-45
TCGCGAT23001.1336633E-619.78355446-47
CGCTAGT17500.001428932417.33644548-49
TTAGCGC23503.0114597E-517.21072244-45
TTCGCGT17650.001514623217.18634444-45
CGAATTA23953.5387777E-516.89006448-49
TCCGTAT48459.094947E-1216.6961346-47
GTCGTAA30659.574978E-716.4952646-47
GCGCGAA18400.002000430116.48629646-47
GTTGCGA24554.3807573E-516.4746244-45
CGCGTAT9452.935356E-616.05226348-49
TAGTCGA18900.00239319516.0496844-45
CACGCGA19150.002612511215.84015544-45
TATCCGT28809.68604E-615.79890444-45
ATCGCAT32051.5259284E-615.7747246-47
CGCGTAG20200.003724118715.01919748-49
Produced by FastQC (version 0.11.2)