FastQCFastQC Report
Sun 7 Aug 2016
GBM12.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM12.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences635321
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCGGT1400.043.4873126
TCGTGCG1201.8189894E-1242.9297837
TATCCGG1450.041.9877475
AGACGTG901.3551471E-841.6255264
CGGTAAA1500.040.5881589
CGCCCTT1051.1768861E-940.1453631
TCAGACG952.183333E-839.434712
GATCGGA609.002592E-539.0302161
TAGCATA3050.038.3872878
TTATCCG1600.038.0483364
GCCAGTA3250.037.4629753
CCGGTAA1650.036.8983238
TCCGGTA1650.036.8983237
CCAGTAG3300.036.8953554
ACGAGCG1204.3528416E-935.121543
TTACGAC1750.034.789855
GTTACGA1750.034.787054
ACGTGGC1107.931703E-834.059996
GTAGCAT3600.033.823467
CAGTAGC3700.032.9093175
Produced by FastQC (version 0.11.2)