FastQCFastQC Report
Sun 7 Aug 2016
GBM12.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM12.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences635321
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGCG1150.057.116787
AGACGGC2350.051.9085669
CCGGAAA851.4733814E-1049.595034
GACCGGA804.76939E-946.8471342
TGTTACG1800.041.6418953
TTACGAC1800.041.6386155
CATACGA950.039.45643214-15
GTTACGA1900.039.4440044
AATCGGA608.990505E-539.039282
GGCTCGT1800.039.0331274
GCTCGTG1700.038.5769545
ATCGGGA1359.094947E-1238.1717382
AGGCGTA1600.038.1203548
GAATCGG500.001725553737.4924741
TCGGAAA1900.036.9787524
CGGAAAG2200.036.1972055
ATACGAG1100.034.11902216-17
GTGCGTC2000.032.842159
TCGGAGA1001.4479629E-632.7878274
GATCGGA1606.184564E-1132.2074052
Produced by FastQC (version 0.11.2)