FastQCFastQC Report
Sun 7 Aug 2016
GBM14.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM14.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33448665
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG32950.013.1340983
CCCGTAT47300.010.6424691
CTCGGTT57800.010.4184211
TTCGCGC27300.010.1655887
CGCGTAA13950.010.1173511
CTCCGAT62850.09.5813021
ATCGAAG111450.09.2853336
GTATCGA38050.09.2717044
CTCGGAT91600.09.2447851
CCGTATC51550.09.0341242
CTCGAAT79350.09.0119031
TCGATCG43300.08.7991548
CTGGAAT382150.08.7652831
TGCGCGA31750.08.7408199
CTCAGAT240300.08.7317421
CCGTACG39900.08.7239214
CTCAAAT255350.08.4566161
CTCGCAT102850.08.3250511
CTTGGAT238500.08.1664211
CGATCGG45550.08.1579789
Produced by FastQC (version 0.11.2)