FastQCFastQC Report
Sun 7 Aug 2016
GBM14.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM14.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33448665
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG39050.011.7863993
TCGATCG46100.011.1091858
CTCAGAT241100.010.403231
CGATCGG49450.010.1665619
CGCGAAT11750.09.9936991
CTCGGTT64300.09.9346391
CTCGGAT88950.09.8746061
CCCGTAT51300.09.7969431
CTCGTAT37850.09.6794991
CTTGGAT251950.09.5823691
CTCGCAT102900.09.4032061
CCGTACG44000.09.3930384
ATCGAAG117650.09.3050916
TGCGCGA30100.09.2096299
CTCCGAT70150.09.173121
CTGGAAT374900.08.9831331
GTATCGA39100.08.7684374
CTCGAAT76100.08.7027921
GCGTACG35900.08.70146294-95
CGTACGC48300.08.5566265
Produced by FastQC (version 0.11.2)