FastQCFastQC Report
Sun 7 Aug 2016
GBM15.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM15.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53242722
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG37750.011.466923
CCCGTAT71000.010.339941
CTCGGAT138850.09.6594121
CGCGTAT17500.09.1430831
CTGGAAT592950.09.11121
CCGGGAT146600.08.6993561
CTCGGTT99500.08.6552531
CTCGCAT106950.08.6243641
TACCGTA48800.08.5807989
CTTGGAT403150.08.439631
CTCAGAT403850.08.3667371
GTATCGA60700.08.2939534
CTCGGGT133700.08.2363771
CTCCGAT108850.08.214421
CCCGGAT143150.07.88990261
CCGTATC74100.07.81073242
CTCGTTT115800.07.80269151
CTCGAAT123100.07.798731
CCGGTAT56900.07.7744081
AATCGGG79950.07.76825765
Produced by FastQC (version 0.11.2)