FastQCFastQC Report
Sun 7 Aug 2016
GBM15.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM15.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53242722
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG39250.011.4888653
CTCGGAT142950.010.7133661
CCCGTAT72050.010.0410521
GACGCTA49250.09.9191184
CTCCGAT115350.09.3263211
CTCAGAT408750.09.19442751
CTTGGAT401800.09.0728611
CTGGAAT588000.08.8442861
GTATCGA61400.08.7213244
CTCGTAT60700.08.5906641
CTCGAAT126050.08.4973691
CTCAAAT436400.08.4504021
TACCGTA45550.08.35623559
CTCGGGT137200.08.3204081
CTCGGTT97700.08.31848051
CGCGAAT18700.08.2901961
CAATCGG65300.08.2004494
ACGCTAC58350.07.96939235
CTCCGGT115150.07.95542051
CTCGCAT109200.07.91566941
Produced by FastQC (version 0.11.2)