FastQCFastQC Report
Sun 7 Aug 2016
GBM22.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM22.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2087784
Sequences flagged as poor quality0
Sequence length101
%GC55

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCA63530.3042939307897752No Hit
AGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCATAG50280.2408295110988493No Hit
GAACAATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCG36060.17271901690979527No Hit
CTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGC32390.15514057009728976No Hit
CAGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCATA27060.1296111091952041No Hit
CTGGCTTGTGGCGGCCAAGCGTTCATAGCGACGTCGCTTTTTGATCCTTC26530.12707253240756708No Hit
GTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATCGAAGGATCAAAA25720.12319282071325385No Hit
GTGAAAGCGGGGCCTCACGATCCTTCTGACCTTTTGGGTTTTAAGCAGGA23370.11193686703222172No Hit
CCCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGA22610.1082966437141007No Hit
CACGATCCTTCTGACCTTTTGGGTTTTAAGCAGGAGGTGTCAGAAAAGTT22030.10551857855027148No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGACT255.614003E-575.0546651
TTGCGAT200.002179887870.363751
CTCGTGA250.0052768856.2909971
CGTGATA250.00527938756.2842453
TCGTGCG1550.054.4660077
CGGAAAC1800.049.5152321
TGCCGTA3700.049.4412352
CTGCCAG3850.048.7367941
CTAATCA5150.047.59114594-95
CAGTTTT9400.047.402513
TCAGACG8150.047.1935542
CTGTCGA405.732187E-446.909171
CCGGCAT1300.046.909171
CTCGGTT503.0448824E-546.9091641
CTGCCGT4050.046.330041
CCGAAAG5300.045.139011
TGTTACG3900.044.4982263
CGCTTAT652.8266077E-643.2934955
TCCCGTC4050.042.8522032
GGCTCGT2100.042.4344944
Produced by FastQC (version 0.11.2)