FastQCFastQC Report
Sun 7 Aug 2016
GBM34.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM34.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78361977
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG59450.010.8431523
CTCGGAT202300.010.21254351
CCCGTAT104500.09.7275341
CTCGGTT143500.09.051541
CTGGAAT846850.08.6804021
CTCAGAT597200.08.5974611
CTTGGAT575250.08.557371
CTCCGAT157250.08.4097091
GTATCGA83300.08.3034414
CCCGGAT218000.08.0306691
CTCAAAT592500.07.942861
CTCGTTT162850.07.8604321
CTCGCAT165500.07.76300571
CTCGAAT160900.07.6924561
CCCGAAT180900.07.6744761
CTCGAAC225600.07.6141081
CCGGAAT151000.07.57346441
CGCCAAT108850.07.5661491
TCGGATT112900.07.41848332
CTGAAAT819350.07.38072351
Produced by FastQC (version 0.11.2)