FastQCFastQC Report
Sun 7 Aug 2016
GBM34.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM34.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78361977
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT107050.010.6650831
CGTATCG64050.010.04838853
CTCGGAT206700.010.0240371
CTCGTAT81250.09.4834221
CTCGGTT145950.09.2711371
CTGGAAT872050.08.9759041
CTCAGAT599950.08.9745811
CTCGCAT159450.08.8692441
CTCCGAT160150.08.7424651
GTATCGA87600.08.6877224
CTTGGAT594450.08.6624321
CTCAAAT605700.08.3464021
CCGGGAT222750.08.2049611
CGCCAAT111100.08.0772571
CGCGAAT23400.08.0313531
CTCGAAT164700.08.0159931
CTCGGGT199950.07.9891781
CTGAGAT733300.07.97045851
CTCCGGT179100.07.94861841
CTCGTTT169400.07.904531
Produced by FastQC (version 0.11.2)