FastQCFastQC Report
Sun 7 Aug 2016
GBM38.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM38.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31631370
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG29150.016.2669493
CCCGTAT49250.014.0149221
GTATCGA39150.012.7115164
CCGTATC51700.010.8971772
CTCGGAT77550.010.4747561
CAATCGG42250.09.7786584
CTTGGAT234150.09.3247761
TCAATCG44900.09.3060853
GACGCTA30800.08.9934144
CTCAGAT240050.08.9782271
CTCCGAT65300.08.916371
ATCGAAG80000.08.8638886
CTGGAAT350600.08.8525651
CCCGTCT136500.08.7717691
AATCGGG51350.08.6855135
CTCGTCT112350.08.525831
CTCGACT45550.08.4528871
TATCGAA61100.08.4520845
CTCGGTT57450.08.3366151
TAAGGCG57700.08.3015978
Produced by FastQC (version 0.11.2)