FastQCFastQC Report
Sun 7 Aug 2016
GBM43.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM43.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53527052
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG56600.015.4426693
GTATCGA70900.012.5930694
CCCGTAT90050.012.1614481
TACCGTA55800.011.0321699
CCGTATC90900.011.0113752
CTCGGAT144150.09.618791
TATCGAA103200.09.4712625
ATCGAAG146650.08.8120236
CTACCGT73850.08.5902758
CTCAGAT384650.08.2358271
CTTGGAT383250.08.1800651
GACGCTA57600.07.9951724
CTGGAAT582850.07.814121
CGCGTAA13301.0404619E-97.7747091
CTCGTTT111750.07.61279541
ACTACCG95750.07.6070587
AATCGGG79950.07.5822045
CCCGTCT222800.07.5101381
CTCCGAT110600.07.35197641
CGGGGTT178550.07.3444191
Produced by FastQC (version 0.11.2)